Optimization of Pulsed Electric-Field-Based Total Polyphenols’ Extraction from Elaeagnus pungens ‘Limelight’ Leaves Using Hydroethanolic Mixtures

In this study, the use of pulsed electric field (PEF) for extraction polyphenols from mature Elaeagnus pungens ‘Limelight’ leaves is discussed. Optimization main parameters that affect process was carried out. More specifically, composition solvent (ethanol, water, and mixtures two at a 25% v/v step gradient) PEF-related (i.e., pulse duration, period, intensity) optimized. The obtained extracts were examined their polyphenol content with Folin–Ciocalteu assay individual also assessed high-performance liquid chromatography. PEF compared to extract without PEF, in terms total polyphenols. According results, optimum found be duration 10 μs, period 1000 an intensity 0.85 kV cm−1 after 20 min extraction. 50% (v/v) ethanol/water mixture. prepared under conditions contain 58% more PEF. Moreover, increase up 92% recorded specific Based on above, it evidenced influenced recovery polyphenols, suggesting such should similar studies, order maximize yield